Model based dynamics analysis in live cell microtubule images

Alphan Altinok1, Erkan Kiris2, Austin J. Peck2, Stuart C. Feinstein2, Leslie Wilson2, B. S. Manjunath1, Kenneth Rose1
Department of Electrical and Computer Engineering1,
University of California, Santa Barbara, Santa Barbara, CA 93106
Department of Molecular, Cellular, and Developmental Biology2,
University of California, Santa Barbara, Santa Barbara, CA 93106


Background: The dynamic growing and shortening behaviors of microtubules are central to the fundamental roles played by microtubules in essentially all eukaryotic cells. Traditionally, microtubule behavior is quantified by manually tracking individual microtubules in time-lapse images under various experimental conditions. Manual analysis is laborious, approximate, and often offers limited analytical capability in extracting potentially valuable information from the data. Results: In this work, we present computer vision and machine-learning based methods for extracting novel dynamics information from time-lapse images. Using actual microtubule data, we estimate statistical models of microtubule behavior that are highly effective in identifying common and distinct characteristics of microtubule dynamic behavior. Conclusion: Computational methods provide powerful analytical capabilities in addition to traditional analysis methods for studying microtubule dynamic behavior. Novel capabilities, such as building and querying microtubule image databases, are introduced to quantify and analyze microtubule dynamic behavior.
[PDF] [BibTex]
Alphan Altinok, Erkan Kiris, Austin Peck, Stuart Feinstein, Leslie Wilson, B. S. Manjunath and Kenneth Rose,
BMC Cell Biology, vol. 8, BioMed Central, Jul. 2007.
Node ID: 510 , DB ID: 317 , Lab: VRL , Target: Journal
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